Depart from San Vincenzo on Old Aurelia in a northbound direction.
The mainly flat road passes through Donoratico (7,5 km).
Go straight until you see the small church of San Guido (on the left hand side 14,8 km).
Turn right here onto the famous Cypress Avenue towards Bolgheri which you will reach after 4.7 km, of which the last 2 km are pedaled uphill.
At Bolgheri (18 km) follow the road that passes counterclockwise through the little village, coming back onto Cypress Avenue and continues downhill for 500 m to at the fork go right towards Bibbona.
From here the beautiful road with rises and falls among olive trees and vineyards and leads to Bibbona (24 km): before entering the village, turn right at the sports field and follow the road downhill to the big intersection.
Follow the signs for Casale Marittimo turning right on the “della Camminata” S.P. 19: after the first kilometre uphill, the road overlooks the hills covered with the “Macchia della Magona”(Macchia della Magona woods) and the Casale Marittimo village.
Continue biking for another 2 km the fork in the road, turn left and go uphill for 1 km to Casale (28 km).
Continue uphill towards Guardistallo (31 km) on the “dei Tre Comuni” S.P.28 then downhill to la Valle del Cecina (the Cecina Valley)